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Bovine Rota Virus G Genotypes in Kashmir: by SA Wani et al

PostPosted: Sun Nov 20, 2005 11:44 pm
by vet
Determination of bovine rotavirus G genotypes
in Kashmir, India

S.A. Wani, M.A. Bhat, S.M. Ishaq & M.A. Ashrafi
Rotavirus Laboratory, Division of Veterinary Microbiology & Immunology, SK University of Agricultural
Sciences and Technology of Kashmir, Shuhama, (Alusteng) Srinagar-190006, India

Rotavirus ribonucleic acid (RNA) was extracted from ten faecal samples of
diarrhoeic calves positive for group A rotavirus by enzyme-linked
immunosorbent assay (ELISA). A portion of the extracted RNA was run in
polyacrylamide gel to determine the presence of rotaviral RNA and the rest
subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) to
generate the near full length VP7 gene. Only six samples yielded the desired
product. The amplified products were subjected to G-typing by PCR using a
cocktail of G6, G8 and G10 typing primers. All of the six samples were
characterised as G10 and none of the samples revealed mixed infection by twin
G types. Four samples, despite possessing sufficient rotavirus particles as
revealed by ELISA and polyacrylamide gel electrophoresis, did not yield any
amplified product on RT-PCR. This could be due to non-specific inhibitors of the
PCR reaction, present in the faecal samples, being carried through the extraction
Bovine – G-typing – India – Polymerase chain reaction – Rotavirus.

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